FlowCytoAbsolute Counting + MESF Calibration
Left panelBead / Volumetric
Right panelMESF / MEF
SourceManual or gated
DemoAuto-boot
◆ABSOLUTE COUNTING
METHOD
SOURCEmanual entry
INPUTS
RESULT
Computing…
◆MESF CALIBRATION
CALIBRATION BEAD PEAKS
#Known MESFMeasured MFI
1
2
3
4
5
MESF = 0 → blank bead (excluded from fit)
POPULATION MFIs TO CONVERTmanual entry
CALIBRATION FIT
Fitting…